Inactivation of wheat-germ aspartate transcarbamoylase by the arginine-specific reagent phenylglyoxal
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چکیده
منابع مشابه
Active-site-directed inactivation of wheat-germ aspartate transcarbamoylase by pyridoxal 5'-phosphate.
Treatment of 1 microM wheat-germ aspartate transcarbamoylase with 1 mM-pyridoxal 5'-phosphate caused a rapid loss of activity, concomitant with the formation of a Schiff base. Complete loss of activity occurred within 10 min when the Schiff base was reduced with a 100-fold excess of NaBH4. Concomitantly, one amino group per chain was modified. No further residues were modified in the ensuing 30...
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Elongation factor T, (EF-T,) is inactivated by the arginine-specific reagent 2,3-butanedione. Excess elongation factor T, (EF-T,), the protein substrate for EFT,, protects EF-T, from this inactivation. Borate ion affects the inactivation kinetics in a manner consistent with the formation of an arginine residueebutanedione l borate complex. The butanedione inactivation is rapidly reversible in t...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1986
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2330303